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AuthorEisa-Beygi, Shahram
AuthorBenslimane, Fatiha M.
AuthorEl-Rass, Suzan
AuthorPrabhudesai, Shubhangi
AuthorAbdelrasoul, Mahmoud Khatib Ali
AuthorSimpson, Pippa M.
Authoryalcin, Huseyin C.
AuthorBurrows, Patricia E.
AuthorRamchandran, Ramani
Available date2018-12-19T05:10:51Z
Publication Date2018-09
Publication NameArteriosclerosis, Thrombosis, and Vascular Biologyen_US
CitationEisa-Beygi, S., Benslimane, F. M., El-Rass, S., Prabhudesai, S., Abdelrasoul, M. K. A., Simpson, P. M., ... & Ramchandran, R. (2018). Characterization of endothelial cilia distribution during cerebral-vascular development in zebrafish (Danio rerio). Arteriosclerosis, Thrombosis, and Vascular Biology, 38(12), 2806-2818.
AbstractObjective—Endothelial cells (ECs) sense and respond to flow-induced mechanical stress, in part, via microtubule-based projections called primary cilia. However, many critical steps during vascular morphogenesis occur independent of flow. The involvement of cilia in regulating these stages of cranial vascular morphogenesis is poorly understood because cilia have not been visualized in primary head vessels. The objective of this study was to investigate involvement of cilia in regulating the early stages of cranial vascular morphogenesis. Approach and Results—Using high-resolution imaging of the Tg(kdrl:mCherry-CAAX) y171;(bactin::Arl13b:GFP) zebrafish line, we showed that cilia are enriched in the earliest formed cranial vessels that assemble via vasculogenesis and in angiogenic hindbrain capillaries. Cilia were more prevalent around the boundaries of putative intravascular spaces in primary and angiogenic vessels. Loss of cardiac contractility and blood flow, because of knockdown of cardiac troponin T type 2a (tnnt2a) expression, did not affect the distribution of cilia in primary head vasculature. In later stages of development, cilia were detected in retinal vasculature, areas of high curvature, vessel bifurcation points, and during vessel anastomosis. Loss of genes crucial for cilia biogenesis (ift172 and ift81) induced intracerebral hemorrhages in an EC-autonomous manner. Exposure to high shear stress induced premature cilia disassembly in brain ECs and was associated with intracerebral hemorrhages. Conclusions—Our study suggests a functional role for cilia in brain ECs, which is associated with the emergence and remodeling of the primary cranial vasculature. This cilia function is flow-independent, and cilia in ECs are required for cerebral-vascular stability.
SponsorS. Eisa-Beygi is supported by funds from Kelleigh’s Cause Foundation. R. Ramchandran is also supported by 1R01HL123338,and partly supported by funds from Women’s Health Research Program at the Medical College of Wisconsin. S. Prabhudesai is supported by Department of Pediatrics, and Children’s Research Institute funds to R. Ramchandran. H.C. Yalcin is supported by QNRF (Qatar National Research Fund), National Priority Research Program NPRP 10-0123-170222, and Qatar University internal funds QUUGBRC-2017-3 and QUST-BRC-SPR\2017-1.
PublisherAmerican Heart Association
Subjectarteriovenous malformations
Subjectendothelial cells
Subjectshear stress
TitleCharacterization of Endothelial Cilia Distribution During Cerebral-Vascular Development in Zebrafish (Danio rerio)
Issue Number12
Volume Number38

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