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AuthorNasrallah, Gheyath K.
AuthorDargham, Soha R.
AuthorSahara, Afifah S.
AuthorElsidiq, Malaz S.
AuthorAbu-Raddad, Laith J.
Available date2019-01-15T08:35:48Z
Publication Date2019-01-07
Publication NameJournal of Clinical Virologyen_US
CitationG.K. Nasrallah et al. Performance of Four Diagnostic Assays for Detecting Herpes Simplex Virus Type 2 Antibodies in the Middle East and North Africa. Journal of Clinical Virology. Vol. 111. P.p. 33-38 .
AbstractBackground Assessments of commercial assays in detecting herpes simplex virus type 2 (HSV-2) antibodies have shown variable sensitivity and specificity, and variation in performance by global population. Objective To evaluate performance of four assays in detecting HSV-2 antibodies in a composite Middle Eastern and North African (MENA) population. The assays are two ELISA kits: HerpeSelect® 2 ELISA IgG and Euroimmun Anti-HSV-2 (gG2) ELISA (IgG), and two immunoblot (IB)/Western blot (WB) assays: HerpeSelect® 1 and 2 Immunoblot IgG and Euroimmun Anti-HSV-1/HSV-2 gG2 Euroline-WB (IgG/IgM). Study design Blood specimens were drawn from blood donors between 2013–2016 in Doha, Qatar. Twenty specimens from ten nationalities (Egypt, Iran, Jordan, Lebanon, Pakistan, Palestine, Qatar, Sudan, Syria, and Yemen; total = 200) were randomly selected and tested for HSV-2 antibodies. Results In the six possible assay comparisons, Cohen’s kappa statistics indicated fair to good agreement, ranging between 0.57 (95% CI 0.28-0.86) and 0.69 (95% CI 0.44-0.95). Meanwhile, positive percent agreement ranged between 50.0 (95% CI 18.7–81.3%) and 63.6% (95% CI 30.8–89.1%); negative percent agreement ranged between 97.8% (95% CI 94.4–99.4%) and 99.5% (95% CI 97.0–100.0%); and overall percent agreement ranged between 95.8% (95% CI 91.9–97.9%) and 97.5% (95% CI 94.2–98.9%). The two ELISA kits demonstrated comparable sensitivities and specificities ≥50% and >98%, respectively, with respect to the IB/WB assays. Conclusion The study provided, for the first time, primary data on performance of these assays in diagnosing HSV-2 infection in MENA populations. Findings support comparable performance and utility of these assays, and demonstrate challenges in establishing seropositivity (versus seronegativity).
SubjectMiddle East and North Africa
SubjectCohen’s kappa statistic
SubjectConcordance measures
TitlePerformance of Four Diagnostic Assays for Detecting Herpes Simplex Virus Type 2 Antibodies in the Middle East and North Africa
Volume Number111

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