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AuthorMarei, Hany E S
AuthorLashen, Samah
AuthorFarag, Amany
AuthorAlthani, Asmaa
AuthorAfifi, Nahla
AuthorA, Abd-Elmaksoud
AuthorRezk, Shaymaa
AuthorPallini, Roberto
AuthorCasalbore, Patrizia
AuthorCenciarelli, Carlo
Available date2019-01-22T11:18:25Z
Publication Date2015-07-01
Publication NameJournal of Cellular Physiologyen_US
CitationMarei, H. E., Lashen, S. , Farag, A. , Althani, A. , Afifi, N. , A, A. , Rezk, S. , Pallini, R. , Casalbore, P. and Cenciarelli, C. (2015), Human olfactory bulb neural stem cells mitigate movement disorders in a rat model of Parkinson's disease. J. Cell. Physiol., 230: 1614-1629. doi:10.1002/jcp.24909
AbstractParkinson's disease (PD) is a neurological disorder characterized by the loss of midbrain dopaminergic (DA) neurons. Neural stem cells (NSCs) are multipotent stem cells that are capable of differentiating into different neuronal and glial elements. The production of DA neurons from NSCs could potentially alleviate behavioral deficits in Parkinsonian patients; timely intervention with NSCs might provide a therapeutic strategy for PD. We have isolated and generated highly enriched cultures of neural stem/progenitor cells from the human olfactory bulb (OB). If NSCs can be obtained from OB, it would alleviate ethical concerns associated with the use of embryonic tissue, and provide an easily accessible cell source that would preclude the need for invasive brain surgery. Following isolation and culture, olfactory bulb neural stem cells (OBNSCs) were genetically engineered to express hNGF and GFP. The hNFG-GFP-OBNSCs were transplanted into the striatum of 6-hydroxydopamin (6-OHDA) Parkinsonian rats. The grafted cells survived in the lesion environment for more than eight weeks after implantation with no tumor formation. The grafted cells differentiated in vivo into oligodendrocyte-like (25 ± 2.88%), neuron-like (52.63 ± 4.16%), and astrocyte -like (22.36 ± 1.56%) lineages, which we differentiated based on morphological and immunohistochemical criteria. Transplanted rats exhibited a significant partial correction in stepping and placing in non-pharmacological behavioral tests, pole and rotarod tests. Taken together, our data encourage further investigations of the possible use of OBNSCs as a promising cell-based therapeutic strategy for Parkinson's disease.
SubjectParkinson's disease (PD)
Subjectolfactory bulb neural stem cells
SubjectNeural stem cells
TitleHuman olfactory bulb neural stem cells mitigate movement disorders in a rat model of Parkinson's disease.
Issue Number7
Volume Number230

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