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AuthorVaradharajan, Kavitha
AuthorShanmugakonar, Muralitharan
AuthorDas, Sandra Concepcion
AuthorAl-Naemi, Hamda A.
Available date2019-04-16T10:37:36Z
Publication Date2018
Publication NameAdvances in Microbiologyen_US
Identifierhttp://dx.doi.org/10.4236/aim.2018.812066
CitationVaradharajan, K., Shanmugakonar, M., Das, S.C. and Al-Naemi, H.A. (2018) Gut Microbiota Dysbiosis in Cafeteria Diet Fed Sprague Dawley Rats. Advances in Microbiology, 8, 975-993. https://doi.org/10.4236/aim.2018.812066
ISSN2165-3402
URIhttp://hdl.handle.net/10576/11485
AbstractDiet plays a major role in the body physiology and metabolism. The quantity, nature and stability of the macronutrients present in the diet have a major impact on the composition of gut microbiota. Gut microbiota plays a major role in the body metabolism and leads to obese or lean phenotype. Bacteriodetes, Firmicutes, Proteobacteria and Actinobacteria are the major microbes that inhabit in the region of the gut. We made an attempt to study the effects of Cafeteria (CAF) diets and normal chow diets on diet consumption, weight gain, metabolism and composition of gut microbiota in fecal and cecum samples from three weeks old Sprague Dawley (SD) rats (n = 18/group) using 16S rDNA high throughput sequencing. Results revealed that distinctive diet based phenotypical changes were observed in some of the Cafeteria diet fed rats. Interestingly, some weight gain resistant (WGR) animals in Cafeteria diet fed groups show similar trend like that of control normal chow fed rats. Fecal microbiome analysis indicates that the ratio of Bacteriodetes is higher than the Firmicutes in cecum samples of Cafeteria diet fed rats whereas no significant difference is found in fecal samples of Cafeteria diet fed rats and as well as in control rats. Further analysis of other taxa at the level of family and genus of microbial abundance are also discussed. Our study suggests that contribution of gut microbiota towards obesity is not at the phylum level, and microbiome composition even at the level of species or strain may exert impact on the metabolism of the Cafeteria diet.
Languageen
PublisherScientific Research Publishing
SubjectGut Microbiota
SubjectObesity
SubjectCafeteria Diet
SubjectFeces
SubjectWeight Gain Resistant
SubjectNext Generation Sequencing
TitleGut Microbiota Dysbiosis in Cafeteria Diet Fed Sprague Dawley Rats
TypeArticle
Pagination975-993
Issue Number12
Volume Number8
dc.identifier.essn 2165-3410


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