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AuthorDzinic, Sijana H
AuthorMahdi, Zaid
AuthorBernardo, M Margarida
AuthorVranic, Semir
AuthorBeydoun, Haya
AuthorNahra, Nadine
AuthorAlijagic, Amra
AuthorHarajli, Deanna
AuthorPang, Aaron
AuthorSaliganan, Dan M
AuthorRahman, Abid M
AuthorSkenderi, Faruk
AuthorHasanbegovic, Berisa
AuthorDyson, Gregory
AuthorBeydoun, Rafic
AuthorSheng, Shijie
Available date2019-04-21T05:10:45Z
Publication Date2019-04-19
Publication NamePLoS ONEen_US
Identifierhttp://dx.doi.org/10.1371/journal.pone.0215089
CitationDzinic SH, Mahdi Z, Bernardo MM, Vranic S, Beydoun H, Nahra N, et al. (2019) Maspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma. PLoS ONE 14(4): e0215089. https://doi.org/10.1371/journal.pone.0215089
URIhttp://hdl.handle.net/10576/11497
AbstractBarrett's esophagus (BE) is a predisposing factor of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma (ECA/GEJ Aca). BE patients are stratified and subsequently monitored according to the risk of malignant progression by the combination of endoscopy and biopsy. This study is to evaluate the maspin expression patterns as early diagnostic markers of malignancy in BE patients. Immunohistochemistry (IHC) staining was performed on 62 archival core biopsies from 35 patients, including BE without dysplasia (intestinal metaplasia, IM), BE with low grade dysplasia, BE with high grade dysplasia, carcinoma in situ, and well to poorly differentiated ECA/GEJ Aca (PD-ECA/GEJ Aca). The intensity and the subcellular distribution of immunoreactivity were evaluated microscopically. Statistical analysis was performed using the χ2 and Fisher exact tests. The level of epithelial-specific tumor suppressor maspin protein inversely correlated with the progression from IM to PD-ECA/GEJ Aca. Lesions of each pathological grade could be divided into subtypes that exhibited distinct maspin subcellular distribution patterns, including nuclear only (Nuc), combined nuclear and cytoplasmic (Nuc+Cyt), cytoplasmic only (Cyt) and overall negligible (Neg). The Cyt subtype, which was minor in both IM and dysplasia (approximately 10%), was predominant in ECA/GEJ Aca as early as well-differentiated lesions (more than 50%: p = 0.0092). In comparison, nuclear staining of the tumor suppressor TP53 was heterogeneous in dysplasia, and did not correlate with the differentiation grades of ECA/GEJ Aca. The Cyt subtype of maspin expression pattern in core biopsies of BE patients may serve as a molecular marker for early diagnosis of ECA/GEJ Aca.
SponsorThis work was supported by the NIH grant P30CA022453 (to the Karmanos Cancer Institute with Sheng, S. as a program leader), the Ruth Sager Memorial Fund (to Sheng, S.), the Karmanos Cancer Institute Pilot Project Grant 25S5Z (to Sheng, S.), and the Karmanos Cancer Institute Prostate Cancer Research Pilot Project Grant (to Sheng, S.).
Languageen
PublisherPublic Library of Science
Subjectcancer
Subjectesophageal adenocarcinoma
SubjectGEJ adenocarcinoma
SubjectBarrett esophagus
SubjectMaspin expression
TitleMaspin differential expression patterns as a potential marker for targeted screening of esophageal adenocarcinoma/gastroesophageal junction adenocarcinoma.
TypeArticle
Issue Number4
Volume Number14
dc.identifier.essn 1932-6203


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