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AuthorAlijani, Yasaman
AuthorHosseini, Saeedeh Sadat
AuthorAhmadian, Salman
AuthorBoughattas, Sonia
AuthorEslami, Gilda
AuthorNaderian, Shadi
AuthorAjamein, Vahid
Available date2019-08-20T10:25:47Z
Publication Date2019-06
Publication NameJournal of Arthropod-Borne Diseasesen_US
Identifierhttp://dx.doi.org/10.18502/jad.v13i2.1240
CitationAlijani Y, Hosseini SS, Ahmadian S, Boughattas S, Eslami G, Naderian S, Ajamian V. Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Isolates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran. J Arthropod Borne Dis. 13(2):145-152.
ISSN1735-7179
URIhttp://hdl.handle.net/10576/11695
AbstractBackground: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is necessary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecular analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for finding the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in resistant isolates.
Languageen
PublisherTehran University of Medical Sciences
SubjectLeishmaniasis
SubjectCutaneous
SubjectDrug rsesistance
SubjectAntimony
TitleMolecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Isolates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran
TypeArticle
Pagination145–152
Issue Number2
Volume Number13
dc.identifier.essn 2322-2271


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