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AuthorElgamal, Aya
AuthorAlthani A, Asmaa
AuthorAbd-Elmaksoud, Ahmed
AuthorKassab, Mohammed
AuthorFarag, Amany
AuthorLashen, Samah
AuthorGabr, Mahmoud M
AuthorZakaria, Mahmoud M
AuthorAlissawi, Mohga M
AuthorIsmail, Hossam El-Din Ali
AuthorAbd El Galil, Ahmed
AuthorCaceci, Thomas
AuthorCenciarelli C, Carlo
AuthorMarei, Hany E
Available date2019-09-22T07:25:15Z
Publication Date2019-08
Publication NameAmerican Journal of Stem Cellsen_US
CitationElgamal, Aya & A, Asmaa & Abd-Elmaksoud, Ahmed & Kassab, Mohammed & Farag, Amany & Lashen, Samah & Gabr, Mahmoud & Zakaria, Mahmoud & Alissawi, Mohga & Ismail, Hossam & Galil, Ahmed & Caceci, Thomas & C, Carlo & Marei, Hany. (2019). Xeno-free trans-differentiation of adipose tissue-derived mesenchymal stem cells into glial and neuronal cells. American journal of stem cells. 8. 38-51.
URIhttp://hdl.handle.net/10576/11898
AbstractMesenchymal stem cells (MSCs) are undifferentiated cells that have the ability of self-renewal and trans-differentiation into other cell types. They hold out hope for finding a cure for many diseases. Nevertheless, there are still some obstacles that limit their clinical transplantation. One of these obstacles are the xenogeneic substances added in either proliferation or differentiation media with subsequent immunogenic and infectious transmission problems. In this study, we aimed to replace fetal bovine serum (FBS), the main nutrient source for MSC proliferation with xeno-free blood derivatives. We tested the effect of human activated pure platelet-rich plasma (P-PRP) and advanced platelet-rich fibrin (A-PRF) on the proliferation of human adipose derived-MSCs (AD-MSCs) at different concentrations. For the induction of MSC neural differentiation, we used human cerebrospinal fluid (CSF) at different concentrations in combination with P-PRP to effect xeno-free/species-specific neuronal/glial differentiation and we found that media with 10% CSF and 10% PRP promoted glial differentiation, while media with only 10% PRP induced a neuron-like phenotype.
Languageen
Publishere-Century Publishing
SubjectA-PRF
SubjectAD-MSCs
SubjectCSF
SubjectP-PRP
Subjectneural differentiation
TitleXeno-free trans-differentiation of adipose tissue-derived mesenchymal stem cells into glial and neuronal cells.
TypeArticle
Pagination38-51
Issue Number2
Volume Number8
ESSN2160-4150


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