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AuthorSohail, Muhammad U
AuthorElrayess, Mohamed A
AuthorAl Thani, A A
AuthorAl-Asmakh, M
AuthorYassine, Hadi M
Available date2019-12-15T06:49:33Z
Publication Date2019-12-01
Publication NameMicroorganismsen_US
Identifierhttp://dx.doi.org/10.3390/microorganisms7120645
CitationSohail, M.U.; Elrayess, M.A.; Al Thani, A.A.; Al-Asmakh, M.; Yassine, H.M. Profiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar. Microorganisms 2019, 7, 645.
ISSN2076-2607
URIhttp://hdl.handle.net/10576/12401
AbstractThe present study is designed to compare demographic characteristics, plasma biochemistry, and the oral microbiome in obese ( = 37) and lean control ( = 36) subjects enrolled at Qatar Biobank, Qatar. Plasma hormones, enzymes, and lipid profiles were analyzed at Hamad Medical Cooperation Diagnostic Laboratory. Saliva microbiome characterization was carried out by 16S rRNA amplicon sequencing using Illumina MiSeq platform. Obese subjects had higher testosterone and sex hormone-binding globulin (SHBG) concentrations compared to the control group. A negative association between BMI and testosterone ( < 0.001, r = -0.64) and SHBG ( < 0.001, r = -0.34) was observed. Irrespective of the study groups, the oral microbiome was predominantly occupied by , , and species. A generalized linear model revealed that the Firmicutes/Bacteroidetes ratio (2.25 ± 1.83 vs. 1.76 ± 0.58; corrected -value = 0.04) was higher, and phylum Fusobacteria concentration (4.5 ± 3.0 vs. 6.2 ± 4.3; corrected -value = 0.05) was low in the obese group compared with the control group. However, no differences in microbiome diversity were observed between the two groups as evaluated by alpha (Kruskal-Wallis ≥ 0.78) and beta (PERMANOVA = 0.37) diversity indexes. Certain bacterial phyla (Acidobacteria, Bacteroidetes, Fusobacteria, Proteobacteria, Spirochaetes, and Firmicutes/Bacteroidetes) were positively associated ( = 0.05, r ≤ +0.5) with estradiol, fast food consumption, creatinine, breastfed during infancy, triglycerides, and thyroid-stimulating hormone concentrations. In conclusion, no differences in oral microbiome diversity were observed between the studied groups. However, the Firmicutes/Bacteroidetes ratio, a recognized obesogenic microbiome trait, was higher in the obese subjects. Further studies are warranted to confirm these findings in a larger cohort.
SponsorQatar National Research Fund
Languageen
PublisherMDPI
Subjectdiabetes
Subjectexercise
Subjectmicrobiome
Subjectmicrobe
Subjectinflammation
Subjectimmune response
Subjectobesity
Subjectoxidative stress
TitleProfiling the Oral Microbiome and Plasma Biochemistry of Obese Hyperglycemic Subjects in Qatar
TypeArticle
Issue Number12
Volume Number7
ESSN2076-2607


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