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AuthorA, Mariadoss
AuthorSatdive, Ramesh
AuthorFulzele, Devanand P.
AuthorRamamoorthy, Siva
AuthorC, George Priya Doss
AuthorZayed, Hatem
AuthorYounes, Salma
AuthorC, Rajasekaran
Available date2019-12-22T08:47:25Z
Publication Date2019-11-21
Publication NameIndustrial Crops and Productsen_US
CitationMariadoss A, et al., Industrial Crops & Products,
IdentifierArticle #: 111987
AbstractThe aim of this study was to obtain high-yielding cell cultures of Rubia cordifolia by applying gamma irradiation and subsequently scaling up for anthraquinone production in a bioreactor. Calli cultured on MS medium was irradiated at variable doses between 2 Gy and 30 Gy. The callus cultures that were irradiated at 8 Gy accumulated a maximum alizarin level of 26.86 mg g-1 DW and a purpurin level of 44.85 mg g-1 DW during the M1T4 (fourth sub-cultures after gamma irradiation treatment) subculture, which was 6-fold and 11-fold higher than those of the nonirradiated callus cultures, respectively. Suspension cultures that originated from high-yielding callus cultures irradiated at 8 Gy were scaled up in an 8-L stirred bioreactor equipped with different impellers. The callus cultures in a bioreactor using a helical ribbon for homogenous mixing synthesized 63.58 % more anthraquinones than those in the bioreactor equipped with a Rushton disc turbine. This is the first report on the production of alizarin and purpurin in a bioreactor.
SubjectRubia cordifolia
SubjectGamma irradiation
SubjectRushton disc turbine
SubjectHelical ribbon impeller
TitleEnhanced production of anthraquinones by gamma-irradiated cell cultures of Rubia cordifolia in a bioreactor

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