Show simple item record

AuthorRakib, Fazle
AuthorAli, Carmen M
AuthorYousuf, Mohammed
AuthorAfifi, Mohammed
AuthorBhatt, Pooja R
AuthorUllah, Ehsan
AuthorAl-Saad, Khalid
AuthorAli, Mohamed H M
Available date2019-12-31T10:29:07Z
Publication Date2019-10-01
Publication NameBrain Sciencesen_US
Identifierhttp://dx.doi.org/10.3390/brainsci9110293
CitationRakib, F.; Ali, C.M.; Yousuf, M.; Afifi, M.; Bhatt, P.R.; Ullah, E.; Al-Saad, K.; Ali, M.H.M. Investigation of Biochemical Alterations in Ischemic Stroke Using Fourier Transform Infrared Imaging Spectroscopy—A Preliminary Study. Brain Sci. 2019, 9, 293.
URIhttp://hdl.handle.net/10576/12417
AbstractBrain damage, long-term disability and death are the dreadful consequences of ischemic stroke. It causes imbalance in the biochemical constituents that distorts the brain dynamics. Understanding the sub-cellular alterations associated with the stroke will contribute to deeper molecular understanding of brain plasticity and recovery. Current routine approaches examining lipid and protein biochemical changes post stoke can be difficult. Fourier Transform Infrared (FTIR) imaging spectroscopy can play a vital role in detecting these molecular alterations on a sub-cellular level due to its high spatial resolution, accuracy and sensitivity. This study investigates the biochemical and molecular changes in peri-infract zone (PIZ) (contiguous area not completely damaged by stroke) and ipsi-lesional white matter (WM) (right below the stroke and PIZ regions) nine weeks post photothrombotic ischemic stroke in rats. FTIR imaging spectroscopy and transmission electron microscopy (TEM) techniques were applied to investigate brain tissue samples while hematoxylin and eosin (H&E) stained images of adjacent sections were prepared for comparison and examination the morphological changes post stroke. TEM results revealed shearing of myelin sheaths and loss of cell membrane, structure and integrity after ischemic stroke. FTIR results showed that ipsi-lesional PIZ and WM experienced reduction in total protein and total lipid content compared to contra-lesional hemisphere. The lipid/protein ratio reduced in PIZ and adjacent WM indicated lipid peroxidation, which results in lipid chain fragmentation and an increase in olefinic content. Protein structural change is observed in PIZ due to the shift from random coli and α-helical structures to β-sheet conformation. FTIR imaging bio-spectroscopy provide novel biochemical information at sub-cellular levels that be difficult to be obtained by routine approaches. The results suggest that successful therapeutic strategy that is based on administration of anti-oxidant therapy, which could reduce and prevent neurotoxicity by scavenging the lipid peroxidation products. This approach will mitigate tissue damage in chronic ischemic period. FTIR imaging bio-spectroscopy can be used as a powerful tool and offer new approach in stroke and neurodegenerative diseases research.
Languageen
PublisherMDPI
SubjectFourier transform infrared (FTIR) imaging spectroscopy
Subjectphotothrombotic stroke
Subjecttransmission electron microscope (TEM), brain ischemia
TitleInvestigation of Biochemical Alterations in Ischemic Stroke Using Fourier Transform Infrared Imaging Spectroscopy-A Preliminary Study.
TypeArticle
Issue Number11
Volume Number9
ESSN2076-3425


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record