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AuthorKveton F.
AuthorBlsakova A.
AuthorLorencova L.
AuthorJerigova M.
AuthorVelic D.
AuthorBlixt O.
AuthorJansson B.
AuthorKasak P.
AuthorTkac J.
Available date2020-04-01T06:50:39Z
Publication Date2019
Publication NameSensors (Switzerland)
ISSN14248220
URIhttp://dx.doi.org/10.3390/s19245409
URIhttp://hdl.handle.net/10576/13580
AbstractThe study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte—a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L−1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis.
SponsorThe financial support received from the Slovak Scientific Grant Agency VEGA 2/0137/18 and 2/0090/16 from the Slovak Research and Development Agency APVV 17-0300 is acknowledged. This publication is the result of the project implementation: Centre for materials, layers and systems for applications and chemical processes under extreme conditions—Stage I, ITMS no.: 26240120007, supported by the ERDF. This publication was supported by Qatar University Collaborative Grant QUCG-CAM-19/20-2. The findings achieved herein are solely the responsibility of the authors.
Languageen
PublisherMDPI AG
SubjectBiosensor
SubjectElectrochemistry
SubjectGlycan
SubjectGraphene screen-printed electrodes
SubjectTn antigen
TitleA graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody
TypeArticle
Issue Number24
Volume Number19


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