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AuthorMarei, Hany E.
AuthorAlthani, Asma
AuthorAfifi, Nahla
AuthorMichetti, Fabrizio
AuthorPescatori, Mario
AuthorPallini, Roberto
AuthorCasalbore, Patricia
AuthorCenciarelli, Carlo
AuthorSchwartz, Philip
AuthorAhmed, Abd-Elmaksoud
Available date2015-10-28T10:43:52Z
Publication Date2011-12-07
Publication NamePLoS ONE
CitationMarei HES, Althani A, Afifi N, Michetti F, Pescatori M, et al. (2011) Gene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra. PLoS ONE 6(12): e28420.
ISSN1932-6203
URIhttp://dx.doi.org/10.1371/journal.pone.0028420
URIhttp://hdl.handle.net/10576/3602
AbstractNeural stem cells (NSC) with self-renewal and multipotent properties serve as an ideal cell source for transplantation to treat neurodegenerative insults such as Parkinson’s disease. We used Agilent’s and Illumina Whole Human Genome Oligonucleotide Microarray to compare the genomic profiles of human embryonic NSC at a single time point in culture, and a multicellular tissue from postmortem adult substantia nigra (SN) which are rich in dopaminergic (DA) neurons. We identified 13525 up-regulated genes in both cell types of which 3737 (27.6%) genes were up-regulated in the hENSC, 4116 (30.4%) genes were up-regulated in the human substantia nigra dopaminergic cells, and 5672 (41.93%) were significantly up-regulated in both cell population. Careful analysis of the data that emerged using DAVID has permitted us to distinguish several genes and pathways that are involved in dopaminergic (DA) differentiation, and to identify the crucial signaling pathways that direct the process of differentiation. The set of genes expressed more highly at hENSC is enriched in molecules known or predicted to be involved in the M phase of the mitotic cell cycle. On the other hand, the genes enriched in SN cells include a different set of functional categories, namely synaptic transmission, central nervous system development, structural constituents of the myelin sheath, the internode region of axons, myelination, cell projection, cell somata, ion transport, and the voltage-gated ion channel complex. Our results were also compared with data from various databases, and between different types of arrays, Agilent versus Illumina. This approach has allowed us to confirm the consistency of our obtained results for a large number of genes that delineate the phenotypical differences of embryonic NSCs, and SN cells.
SponsorScience and Technology Development Fund (STDF, Egypt: ID:99).
Languageen
PublisherPublic Library of Science
SubjectGene Expression Profiling
SubjectEmbryonic Human Neural Stem Cells
TitleGene Expression Profiling of Embryonic Human Neural Stem Cells and Dopaminergic Neurons from Adult Human Substantia Nigra
TypeArticle
Issue Number12
Volume Number6


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