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AuthorSaadaoui, Imen
AuthorAl-Thani, Roda
AuthorAl-Saadi, Fatma
AuthorHassan, Najeh Belguith-Ben
AuthorAbdelkefi-Mesrati, Lobna
AuthorSchultz, Patrick
AuthorRouis, Souad
AuthorJaoua, Samir
Available date2015-12-08T11:08:17Z
Publication Date2010-12
Publication NameCurrent Microbiology
CitationSaadaoui, I., Al-Thani, R., Al-Saadi, F., Hassan, N.B.-B., Abdelkefi-Mesrati, L., Schultz, P., Rouis, S., Jaoua, S. Characterization of tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect ephestia kuehniella. (2010) Current Microbiology, 61 (6), pp. 541-548. First available online April 2010.
ISSN0343-8651
ISSN1432-0991
URIhttp://dx.doi.org/10.1007/s00284-010-9650-1
URIhttp://hdl.handle.net/10576/3925
AbstractThe study of 257 crystal-producing Bacillus thuringiensis isolates from bioinsecticide free soil samples collected from different sites in Tunisia, was performed by PCR amplification, using six primer pairs specific for cry1, cry2, cry3, cry4, and vip3A genes, by the investigation of strain plasmid pattern, crystal morphology and delta-endotoxin content and by the assessment of insecticidal activities against the lepidopteran insect Ephestia kuehniella. Based on plasmid pattern study, 11 representative strains of the different classes were subjected to morphological and molecular analyses. The comparison of the PFGE fingerprints confirmed the heterogeneity of these strains. B. thuringiensis kurstaki strains, harbouring at the same time the genes cry1A, cry2, cry1Ia, and vip3A, were the most abundant (65.4%). 33.34% of the new isolates showed particular delta-endotoxin profiles but no PCR products with the used primer sets. B. thuringiensis israelensis was shown to be also very rare among the Tunisian B. thuringiensis isolates diversity. These findings could have considerable impacts for the set up of new pest control biological agents.
SponsorTunisian Ministry of Higher Education, Scientific Research and Technology, the National Priorities Research Program (NPRP) of Qatar National Research Fund number 27-6-7-24, the AUF Agence Universitaire de la Francophonie and the NEPAD New Partnership for Africa Development
Languageen
PublisherSpringer US
SubjectBacillus thuringiensis
Subjectephestia kuehniella
SubjectLepidoptera
SubjectBacillus thuringiensis
SubjectEphestia kuehniella
TitleCharacterization of tunisian Bacillus thuringiensis strains with abundance of kurstaki subspecies harbouring insecticidal activities against the lepidopteran insect ephestia kuehniella
TypeArticle
Pagination541-548
Issue Number6
Volume Number61


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