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AuthorBen Hamadou-Charfi, Dorra
AuthorSauer, Annette Juliane
AuthorAbdelkefi-Mesrati, Lobna
AuthorTounsi, Slim
AuthorJaoua, Samir
AuthorStephan, Dietrich
Available date2015-12-22T05:51:36Z
Publication Date2015-04-06
Publication NamePreparative Biochemistry and Biotechnologyen_US
CitationDorra Ben Hamadou-Charfi , Annette Juliane Sauer , Lobna Abdelkefi- Mesrati , Slim Tounsi , Samir Jaoua & Dietrich Stephan (2015) "Susceptibility of Agrotis segetum (noctuidae) to Bacillus thuringiensis and Analysis of Midgut Proteinases", Preparative Biochemistry and Biotechnology, 45:5, 411-420
ISSN1082-6068
ISSN1532-2297 (online)
URIhttp://dx.doi.org/10.1080/10826068.2014.923449
URIhttp://hdl.handle.net/10576/3970
AbstractSeventy-eight Bacillus thuringiensis isolates were selected for a screening against the Lepidoptera species Agrotis segetum to search the higher insecticidal activity. In a preliminary bioassay, the spore-crystal mixture of 78 B. thuringiensis isolates was tested against L1 larvae of A. segetum. Fifty-two isolates had more than 60% corrected mortality after 3 days. Seven isolates caused a corrected mortality of 100% on A. segetum. Twelve isolates were selected for a second bioassay investigating the effect of the vegetative insecticidal protein (Vip) against third-instar larvae. After 7 days, the weight gain and the larval stage of each larva were recorded. This bioassay showed an aberration in larval growth increases, morphology, and weight gain. After plasmid pattern analysis, the most active strains are most likely B. thuringiensis kurstaki strains expressing the Vip3A toxin. The absence of two proteinase activities observed in the case of Cry1Ac would be the consequence of the difference in susceptibility of A. segetum to the toxins used.
SponsorJKI Darmstadt, Germany, and the CBS Sfax, Tunisia.
Languageen
PublisherTaylor & Francis
SubjectAgrotis segetum
SubjectBacillus thuringiensis
SubjectBioassays
SubjectBiochemical characterization
SubjectVegetative insecticidal proteins
Subjectzymogram
TitleSusceptibility of Agrotis segetum (noctuidae) to Bacillus thuringiensis and analysis of midgut proteinases
TypeArticle
Pagination411-420
Issue Number5
Volume Number45


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