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AuthorJallouli, Wafa
AuthorJaoua, Samir
AuthorZouari, Nabil
Available date2015-12-30T11:05:24Z
Publication Date2012-11
Publication NameBioprocess and Biosystems Engineeringen_US
CitationJallouli, W., Jaoua, S., & Zouari, N., (2012), "Improvement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch fermentations optimization", 35(9): 1505–1513
ISSN1615-7605 (online)
AbstractOptimization of a fermentation process for bioinsecticides production by Photorhabdus temperata strain K122 was investigated into fully controlled 3-L fermenter using an optimized medium (OM). Development of large-scale inocula showed that the composition of the growth medium greatly influenced the physiological state of P. temperata cells. The effect of pH, agitation and dissolved oxygen concentration (DO) on the growth, culturability and oral toxicity of P. temperata cells were also investigated. Indeed, maintaining the pH at 7 and controlling DO concentration at 50 % saturation throughout the fermentation process, improved biomass production, CFU counts and oral toxicity by 41.1, 35 and 32.1 %, respectively, as compared to cultures carried out in 500 mL shake flasks. At such conditions, 8 g/L glucose fed-batch fermentation, enhanced cell lysis and variants small colony (Vsm) polymorphism appearance. To overcome such limitations, glucose concentration should be maintained at 4 g/L. In this case, P. temperata cells were produced at high cell density and culturability reaching 4.5 and 1.2 × 10(9) cells/mL, respectively. In addition, the stability of the primary form was maintained for a long period in the stationary growth phase and Vsm polymorphism was completely avoided that can be crucial for scale-up the bioprocess of P. temperata bioinsecticide.
SponsorTunisian Ministere de l’Enseignement Superieur, de la Recherche Scientifique (MESRS)
SubjectPhotorhabdus temperata K122
SubjectDissolved oxygen concentration
SubjectFed-batch fermentation
SubjectPhotorhabdus/growth & development
SubjectPest Control, Biological/methods
TitleImprovement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch fermentations optimization
Issue Number9
Volume Number35

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