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AuthorMohamed, T.
AuthorBen Hsouna, Anis
AuthorHammami, Inès
AuthorCulioli, Gérald
AuthorKsantini, Mohieddine
AuthorTounsi, Slim
AuthorJaoua, Samir
Available date2016-01-06T13:57:04Z
Publication Date2013-03
Publication NameCrop Protectionen_US
Identifierhttp://dx.doi.org/ 10.1016/j.cropro.2012.11.014
CitationTrigui M. , Ben Hsouna A. , Hammami I. , Culioli G. , Ksantini M., Tounsi S. & Jaoua S. (2013), “Efficacy of Lawsonia inermis leaves extract and its phenolic compounds against olive knot and crown gall diseases” , Crop Protection, Vol. 45, pp. 83-88
ISSN0261-2194
URIhttp://hdl.handle.net/10576/4068
AbstractThis study was undertaken to determine the antibacterial efficacy of the leaves extracts of Lawsonia inermis in vitro and in vivo, against the phytopathogenic bacteria Pseudomonas savastanoi pv savastanoi IVIA 1628 and Agrobacterium tumefaciens. The hydroalcoholic extract of L. inermis was fractionated by liquid–liquid partition using hexane, chloroform, ethyl acetate and butanol. Among the tested fractions, the ethyl acetate (EtOAcF) and chloroform (CHCl3F) extracts exhibited high inhibition against all tested plant pathogens. The extracts exerted a bactericidal activity against both P. savastanoi and A. tumefaciens with MIC values ranging from 1.25 to 2.5 mg/ml. In planta experiments, the EtOAcF, tested at four concentrations (0.4, 0.2, 0.1 and 0.05 mg/wounds), completely inhibited the formation of knots on twigs of olive and tomato plants inoculated with pathogenic strains of P. savastanoi and A. tumefaciens (strains B6 and C58). The phytochemical screening revealed that the L. inermis fractions contain flavonoids, quinones, tannins and terpenoids. The qualitative analysis of the EtOAcF by LC–DAD–ELSD–ESI/MSn showed the presence of eight identified phenolic and phenolic glycoside compounds. These results suggest that L. inermis extracts could be used to control plant bacterial diseases caused by P. savastanoi and A. tumefaciens.
Languageen
PublisherElsevier
SubjectLawsonia inermis
SubjectAgrobacterium tumefaciens
SubjectPseudomonas savastanoi pv savastanoi
SubjectBiological control
SubjectAntibacterial activity
TitleEfficacy of Lawsonia inermis leaves extract and its phenolic compounds against olive knot and crown gall diseases.
TypeArticle
Pagination83–88
Volume Number45


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