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AuthorAbuOdeh, Raed O.
AuthorAl-Absi, Enas
AuthorAli, Nadima H.
AuthorKhalili, Makiyeh
AuthorAl-Mawlawi, Naema
AuthorHadwan, Tameem A.
AuthorAl Thani, Asmaa A.
AuthorNasrallah, Gheyath K.
Available date2016-01-12T13:06:56Z
Publication Date2015-12
Publication NameJournal of Medical Virologyen_US
CitationAbuOdeh, R. O., Al-Absi, E., Ali, N. H., Khalili, M., Al-Mawlawi, N., Hadwan, T. A., Althani, A. A. and Nasrallah, G. K. (2015), "Detection and phylogenetic analysis of human pegivirus (GBV-C) among blood donors and patients infected with hepatitis B virus (HBV) in Qatar". Journal of Medical Virology, 87: 2074–2081.
ISSN0146-6615
ISSN1096-9071 (online)
URIhttp://dx.doi.org/10.1002/jmv.24289
URIhttp://hdl.handle.net/10576/4074
AbstractHuman Pegivirus (HPgV), formerly GB virus-C/ Hepatitis G virus (GBV-C/HGV), collectively known as GBV-C, is widely spread and has been reported to be associated with non-A–E hepatitis. To our knowledge, no previous study was conducted about HPgV in Qatar. Thus, the objectives of this study were as follows: (i) to determine the rates of HPgV infection in Qatar among healthy blood donors and HBV-infected patients, and (ii) to determine the most predominant HPgV genotype in Qatar. A total of 714 blood plasma samples from healthy donors (612) and HBV-infected patients (102) were collected. RNA was extracted, reversed transcribed, and then subjected for HPgV detection by two round-nested PCR using primers amplifying a 208 bp of 50-UTR of the HPgV. For genotyping, the 50-UTR PCR products (from 25 randomly picked samples) were cloned and sequenced. The overall infection rate of HPgV in Qatar was 13.3%. There was no significant difference (P¼0.41) in the infection rates between healthy donor (13.7%) and in HBVinfected patients (10.7%). Moreover, we did not find any significant association between HPgV infection rates and nationality, sex, or age (P>0.05). Sequence analysis of 40 50-UTR PCR amplicons yielded the European genotype 2 as most predominant in Qatar, although other genotypes (5 and7) were also present. Our results indicate that there is no strong correlation between HPgV infection rate, condition, nationality, age, and sex, and genotype 2 is most predominant in Qatar
SponsorQatar University. Grant Number: QUST-CAS-FALL-12/13-1
SponsorHamad Medical Center Medical Research Committee. Grant Number: Protocol #13204/13
SponsorBiomedical Research Center
Languageen
PublisherWiley
Subjecthealthy
SubjectHPgV
SubjectHBV
SubjectRT-PCR reaction
Subject50-UTR
TitleDetection and Phylogenetic Analysis of Human Pegivirus (GBV-C) Among Blood Donors and Patients Infected With Hepatitis B Virus (HBV) in Qatar
TypeArticle
Pagination2074-2081
Issue Number12
Volume Number87


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