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AuthorOliva-Olivera, Wilfredo
AuthorCoin-Araguez, Leticia
AuthorSalas, Julian
AuthorLhamyani, Said
AuthorGentile, Adriana-Mariel
AuthorGarcia, Esteban Sarria
AuthorHmadcha, Abdelkrim
AuthorZayed, Hatem
AuthorVega-Rioja, Antonio
AuthorTinahones, Francisco J.
AuthorEl Bekay, Rajaa
Available date2016-03-31T14:00:57Z
Publication Date2015-12
Publication NamePLoS ONE
CitationOliva-Olivera W, Coin-Araguez L, Salas J, Lhamyani S, Gentile A-M, Sarria Garcia E, et al. (2015) Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE 10(12): e0144401.
ISSN1932-6203
URIhttp://dx.doi.org/10.1371/journal.pone.0144401
URIhttp://hdl.handle.net/10576/4293
AbstractObjective Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. Material and Methods We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. Results TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. Conclusions This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
SponsorInstituto de Salud Carlos III/FEDER, EU (PI10/01947, PI13/02628), CTS-7895 from the Consejer?a de Econom?a e Innovaci?n, Ciencia y Empleo, Junta de Andaluc?a/FEDER, EU. R. El Bekay is supported by fellowships from the ISCIII/FEDER, EU "Miguel Servet II" (CPII13/00041). AV-R is under a contract Proyectos de I+D+i para j?venes investigadores from the Ministerio de Econom?a y Competitividad (SAF2014-60649-JIN) and co-funded by Fondo Europeo de Desarrollo Regional-FEDER.
Languageen
PublisherPublic Library of Science
Subject5' nucleotidase
SubjectCD133 antigen
SubjectCD14 antigen
SubjectCD19 antigen
SubjectCD34 antigen
SubjectCD45 antigen
Subjectendoglin
SubjectHLA DR antigen
SubjectThy 1 antigen
Subjectadipocyte
Subjectadipogenesis
Subjectadipose tissue
Subjectadipose tissue cell
Subjectadipose tissue stromal cell
Subjectbone development
Subjectcell adhesion
Subjectcell differentiation
Subjectcell structure
Subjectcolony forming unit F
Subjectheart muscle ischemia
Subjectosteocyte
Subjectstroma cell
Subjectsubcutaneous fat
Subjectthymus
Subjectthymus adipose tissue
TitleMyocardial ischemic subject's thymus fat: A novel source of multipotent stromal cells
TypeArticle
Issue Number12
Volume Number10


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