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AuthorMustafa, Ibrahim
AuthorAl Marwani, Asma
AuthorNasr, Khuloud Mamdouh
AuthorKano, Noora Abdulla
AuthorHadwan, Tameem
Available date2016-09-20T10:56:41Z
Publication Date2016-01-03
Publication NameBioMed Research Internationalen_US
Identifierhttp://dx.doi.org/10.1155/2016/4529434
CitationIbrahim Mustafa, Asma Al Marwani, Khuloud Mamdouh Nasr, Noora Abdulla Kano, and Tameem Hadwan, “Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM,” BioMed Research International, vol. 2016, Article ID 4529434, 7 pages, 2016.
ISSN2314-6133
URIhttp://hdl.handle.net/10576/4717
AbstractUsually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2–6°C. However, molecular and biochemical consequences can affect the stored blood cells; these changes are collectively labeled as storage lesions. In this study, the effect of prolonged storage was assessed through investigating morphological changes and evaluating oxidative stress. Samples from leukodepleted pRBC in SAGM stored at 4°C for 42 days were withdrawn aseptically on day 0, day 14, day 28, and day 42. Morphological changes were observed using scanning electron microscopy and correlated with osmotic fragility and hematocrit. Oxidative injury was studied through assessing MDA level as a marker for lipid peroxidation. Osmotic fragility test showed that extended storage time caused increase in the osmotic fragility. The hematocrit increased by 6.6% from day 0 to day 42. The last 2 weeks show alteration in the morphology with the appearance of echinocytes and spherocytes. Storage lesions and morphological alterations appeared to affect RBCs during the storage period. Further studies should be performed to develop strategies that will aid in the improvement of stored pRBC quality and efficacy.
SponsorThis study was supported by ‘Qatar University student grant QUST-CAS-FALL-14\15-23’.The authors are grateful to Dr. Zeyd Merenkov from the Blood Bank of Hamad Medical Cooperation for assisting in this research. The authors also appreciate the efforts of Mr. Essam Attia at Qatar University Central Laboratory for assisting with the electron microscopy. The authors also acknowledge the support of Dr. Gheyath K. Nasrallah’s laboratory to use some of the equipment in his laboratory, at Biomedical Research Centre of Qatar University.
Languageen
PublisherHindawi
SubjectStorage lesions
SubjectErythrocytes
SubjectLipid peroxidation
SubjectGhost cell
TitleTime Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM
TypeArticle
Volume Number2016


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