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AuthorBertok, Tomas
AuthorSediva, Alena
AuthorFilip, Jaroslav
AuthorIlcikova, Marketa
AuthorKasak, Peter
AuthorVelic, Dusan
AuthorJane, Eduard
AuthorMravcová, Martina
AuthorRovenský, Jozef
AuthorKunzo, Pavol
AuthorLobotka, Peter
AuthorŠmatko, Vasilij
AuthorVikartovská, Alica
AuthorTkac, Jan
Available date2016-11-13T10:42:33Z
Publication Date2015-06-30
Publication NameLangmuiren_US
Identifierhttp://dx.doi.org/10.1021/acs.langmuir.5b00944
CitationBertok T, Šedivá A, Filip J, et al. Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum. Langmuir. 2015;31(25):7148-715.
ISSN0743-7463
URIhttp://hdl.handle.net/10576/4991
AbstractImpedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification of a gold surface by a self-assembled monolayer (SAM) deposited from a solution containing a carboxybetaine-terminated thiol applied to the subsequent covalent immobilization of lectins and to resist nonspecific protein adsorption. In the next step, Sambucus nigra agglutinin (SNA) or Ricinus communis agglutinin (RCA) was covalently attached to the SAM, and the whole process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques including electrochemical impedance spectroscopy, cyclic voltammetry, quartz crystal microbalance, contact angle measurements, zeta-potential assays, X-ray photoelectron spectroscopy, and atomic force microscopy. In addition, the application of the SNA-based lectin biosensor in the glycoprofiling of antibodies isolated from the human sera of healthy individuals and of patients suffering from rheumatoid arthritis (RA) was successfully validated using an SNA-based lectin microarray. The results showed that the SNA lectin, in particular, is capable of discriminating between the antibodies isolated from healthy individuals and those from RA patients based on changes in the amount of sialic acid present in the antibodies. In addition, the results obtained by the application of RCA and SNA biosensors indicate that the abundance of galactose and sialic acid in antibodies isolated from healthy individuals is age-related.
SponsorSlovak Scientific Grant Agency VEGA 2/0162/14 and from the Slovak Research and Development Agency APVV 0282-11. The European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement no. 311532. This publication was made possible by NPRP grant no. 6-381-1-078 from the Qatar National Research Fund (a member of the Qatar Foundation).
Languageen
PublisherAmerican Chemical Society
SubjectArthritis, Rheumatoid/blood
SubjectElectrochemistry
SubjectBiosensing Techniques/methods
TitleCarboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum
TypeArticle
Pagination7148-7157
Issue Number25
Volume Number31
ESSN1520-5827


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