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AuthorZghal, Raida Zribi
AuthorElleuch, Jihen
AuthorAli, Mamdouh Ben
AuthorDarriet, Frederic
AuthorRebaï, Ahmed
AuthorChandre, Fabrice
AuthorJaoua, Samir
AuthorTounsi, Slim
Available date2017-02-19T08:47:15Z
Publication Date2017-01
Publication NameApplied Microbiology and Biotechnologyen_US
Identifierhttp://dx.doi.org/10.1007/s00253-016-7766-3
CitationZghal, R.Z., Elleuch, J., Ben Ali, M. et al. "Towards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin" Appl Microbiol Biotechnol (2017) 101: 113.
ISSN0175-7598
URIhttp://hdl.handle.net/10576/5278
AbstractAttempts have been made to express or to merge different Cry proteins in order to enhance toxic effects against various insects. Cry1A proteins of Bacillus thuringiensis form a typical bipyramidal parasporal crystal and their protoxins contain a highly conserved C-terminal region. A chimerical gene, called cry(4Ba-1Ac), formed by a fusion of the N-terminus part of cry4Ba and the C-terminus part of cry1Ac, was constructed. Its transformation to an acrystalliferous B. thuringiensis strain showed that it was expressed as a chimerical protein of 116 kDa, assembled in spherical to amorphous parasporal crystals. The chimerical gene cry(4Ba-1Ac) was introduced in a B. thuringiensis kurstaki strain. In the generated crystals of the recombinant strain, the presence of Cry(4Ba-1Ac) was evidenced by MALDI-TOF. The recombinant strain showed an important increase of the toxicity against Culex pipiens larvae (LC50 = 0.84 mg l−1 ± 0.08) compared to the wild type strain through the synergistic activity of Cry2Aa with Cry(4Ba-1Ac). The enhancement of toxicity of B. thuringiensis kurstaki expressing Cry(4Ba-1Ac) compared to that expressing the native toxin Cry4Ba, might be related to its a typical crystallization properties. The developed fusion protein could serve as a potent toxin against different pests of mosquitoes and major crop plants.
SponsorThis work was supported by grants from the Tunisian Ministry of Higher Education and Scientific Research.
Languageen
PublisherSpringer-Verlag
SubjectBacillus thuringiensis kurstaki
SubjectChimerical protein Cry(4Ba-1Ac)
SubjectToxicity improvement to Culex pipiens
TitleTowards novel Cry toxins with enhanced toxicity/broader: a new chimeric Cry4Ba / Cry1Ac toxin
TypeArticle
Pagination113-122
Issue Number1
Volume Number101
dc.identifier.essn 1432-0614


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