Show simple item record

AuthorYassine, Hadi M
AuthorMcTamney, Patrick M
AuthorBoyington, Jeffery C
AuthorRuckwardt, Tracy J
AuthorCrank, Michelle C
AuthorSmatti, Maria K
AuthorLedgerwood, Julie E
AuthorGraham, Barney S
Available date2018-06-27T08:57:10Z
Publication Date2018-06-01
Publication NameScientific Reportsen_US
Identifierhttp://dx.doi.org/10.1038/s41598-018-26538-7
CitationYassine, Hadi M. McTamney, Patrick M. Boyington, Jeffery C.Ruckwardt, Tracy J. Crank, Michelle C. Smatti, Maria K. Ledgerwood, Julie E. Graham, Barney S. Use of Hemagglutinin Stem Probes Demonstrate Prevalence of Broadly Reactive Group 1 Influenza Antibodies in Human Sera. Scientific Reports. (2018) 8:8628 . DOI:10.1038/s41598-018-26538-7
ISSN2045-2322
IdentifierArticle number: 8628 (2018)
URIhttp://hdl.handle.net/10576/6765
AbstractA better understanding of the seroprevalence and specificity of influenza HA stem-directed broadly neutralizing antibodies (bNAbs) in the human population could significantly inform influenza vaccine design efforts. Here, we utilized probes comprising headless, HA stabilized stem (SS) to determine the prevalence, binding and neutralization breadth of antibodies directed to HA stem-epitope in a cross-sectional analysis of the general population. Five group-1 HA SS probes, representing five subtypes, were chosen for this analyses. Eighty-four percent of samples analyzed had specific reactivity to at least one probe, with approximately 60% of the samples reactive to H1 probes, and up to 45% reactive to each of the non-circulating subtypes. Thirty percent of analyzed sera had cross-reactivity to at least four of five probes and this reactivity could be blocked by competing with F10 bNAb. Binding cross-reactivity in sera samples significantly correlated with frequency of H1H5 cross-reactive B cells. Interestingly, only 33% of the cross-reactive sera neutralized both H1N1 and H5N1 pseudoviruses. Cross-reactive and neutralizing antibodies were more prevalent in individuals >50 years of age. Our data demonstrate the need to use multiple HA-stem probes to assess for broadly reactive antibodies. Further, a universal vaccine could be designed to boost pre-existing B-cells expressing stem-directed bNAbs.
Languageen
PublisherNature Publishing Group
SubjectInfluenza virus
SubjectViral infection
TitleUse of Hemagglutinin Stem Probes Demonstrate Prevalence of Broadly Reactive Group 1 Influenza Antibodies in Human Sera.
TypeArticle
Volume Number8
ESSN2045-2322


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record