Browsing by Author "Souchelnytskyi, S."
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A protocol for the detection of genetic markers in saliva by polymerase chain reaction without a nucleic acid purifi cation step: Examples of SARS-CoV-2 and GAPDH markers
Kannan, S.; Ericsson, J.; Souchelnytskyi, N.; Souchelnytskyi, S. ( Danylo Halytskyi Lviv National Medical University , 2021 , Article)Introduction. Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs purifi cation step adds time, cost, and aff ects the quality of testing. The objective ... -
Application of nucleic acid amplification tests in managing COVID-19 pandemic
Souchelnytskyi, S.; Souchelnytskyi, N. ( Danylo Halytskyi Lviv National Medical University , 2020 , Article)Background. COVID-19 pandemic highlighted the importance of sensitive and specific tests that would be cost-efficient, fast and scalable. There are more than 200 COVID-19 detection tests available worldwide, with every ... -
Cell-based diagnostic of bronchial carcinoma: Case study of a diagnostic value of tests of circulating tumor cells and organoid culture
Souchelnytskyi, N.; Kost, A.; Souchelnytskyi, S. ( Danylo Halytskyi Lviv National Medical University , 2020 , Article)Diagnostic based on analysis of living tumor cells is frequently used in oncology. Circulating tumor cells in the blood and cells obtained from a tumor biopsy are used to access their carcinogenic properties and subsequently ... -
Exposure to EGF and 17-estradiol irreversibly affects the proliferation and transformation of MCF7 cells but is not sufficient to promote tumor growth in a xenograft mouse model upon withdrawal of exposure
Cunha,Sara,I.; Jia,Min; Souchelnytskyi,Serhiy ( Spandidos Publications , 2018 , Article)Epidermal growth factor (EGF) and estrogen are potent regulators of breast tumorigenesis. Their short‑term actions on human breast epithelial cells have been investigated extensively. However, the consequence of a long‑term ... -
Identification of cortactin molecular forms in human urine and their possible diagnostic value
Starykovych, M.; Souchelnytskyi, S.; Fayura, O.; Abrahamovych, O.; Abrahamovych, M.; Lukavetskyy, N.; Stoika, R.; Kit, Y.... more authors ... less authors ( Palladin Institute of Biochemistry of the NASU , 2021 , Article)The protein composition of human urine reflects changes in the biochemical and physiological status of an individual and has an essential diagnostic value. Using precipitation/extraction methods we isolated a protein with ... -
Proteomics of transforming growth factor ?1 (TGF ?1) signaling in 184A1 human breast epithelial cells suggests the involvement of casein kinase 2? in TGF ?1-dependent p53 phosphorylation at Ser392
Woksepp, H.; Saini, R. K.R.; Zakharchenko, O.; Gautier, A.; Souchelnytskyi, N.; Souchelnytskyi, S.... more authors ... less authors ( Morion LLC , 2019 , Article)Aim: Transforming growth factor ?1 (TGF ?1) is a potent regulator of breast tumorigenesis. It inhibits proliferation of carcinoma cells, but the strength of its inhibitory action varies for cells from benigh, non-metastatic ... -
The purification and identification of human blood serum proteins with affinity to the antitumor active RL2 lactaptin using magnetic microparticles
Manko N.; Starykovych M.; Bobak Y.; Stoika R.; Richter V.; Koval O.; Lavrik I.; Horák D.; Souchelnytskyi S.; Kit Y.... more authors ... less authors ( John Wiley and Sons Ltd , 2019 , Article)The cytopoxic effect of RL2 lactaptin (the recombinant analog of proteolytic fragment of human kappa‐casein) toward tumor cells in vitro and in vivo presents it as a novel promising antitumor drug. The binding of any drug ...