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AuthorBertok T.
AuthorLorencova L.
AuthorHroncekova S.
AuthorGajdosova V.
AuthorJane E.
AuthorHires M.
AuthorKasak P.
AuthorKaman O.
AuthorSokol R.
AuthorBella V.
AuthorEckstein A.A.
AuthorMosnacek J.
AuthorVikartovska A.
AuthorTkac J.
Available date2020-04-09T12:27:28Z
Publication Date2019
Publication NameBiosensors and Bioelectronics
ResourceScopus
ISSN9565663
URIhttp://dx.doi.org/10.1016/j.bios.2019.01.052
URIhttp://hdl.handle.net/10576/13986
AbstractIn this paper several advances were implemented for glycoprofiling of prostate specific antigen (PSA), what can be applied for better prostate cancer (PCa) diagnostics in the future: 1) application of Au nanoshells with a magnetic core (MP@silica@Au); 2) use of surface plasmons of Au nanoshells with a magnetic core for spontaneous immobilization of zwitterionic molecules via diazonium salt grafting; 3) a double anti-fouling strategy with integration of zwitterionic molecules on Au surface and on MP@silica@Au particles was implemented to resist non-specific protein binding; 4) application of anti-PSA antibody modified Au nanoshells with a magnetic core for enrichment of PSA from a complex matrix of a human serum; 5) direct incubation of anti-PSA modified MP@silica@Au with affinity bound PSA to the lectin modified electrode surface. The electrochemical impedance spectroscopy (EIS) signal was enhanced 43 times integrating Au nanoshells with a magnetic core compared to the biosensor without them. This proof-of-concept study shows that the biosensor could detect PSA down to 1.2 fM and at the same time to glycoprofile such low PSA concentration using a lectin patterned biosensor device. The biosensor offers a recovery index of 108%, when serum sample was spiked with a physiological concentration of PSA (3.5 ng mL 1 ).
SponsorEuropean Regional Development Fund, Grantov Agentura ceska Republiky
Languageen
PublisherElsevier Ltd
SubjectAu nanoshells with a magnetic core
Cancer biomarker
Carboxybetaine
Diazonium salt
Impedance spectroscopy
Prostate specific antigen
TitleAdvanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core
TypeArticle
Pagination24-29
Volume Number131


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