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المؤلفJallouli, Wafa
المؤلفJaoua, Samir
المؤلفZouari, Nabil
تاريخ الإتاحة2015-12-30T11:05:24Z
تاريخ النشر2012-11
اسم المنشورBioprocess and Biosystems Engineering
الاقتباسJallouli, W., Jaoua, S., & Zouari, N., (2012), "Improvement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch fermentations optimization", 35(9): 1505–1513
الرقم المعياري الدولي للكتاب1615-7591
الرقم المعياري الدولي للكتاب1615-7605 (online)
معرّف المصادر الموحدhttp://dx.doi.org/10.1007/s00449-012-0740-2
معرّف المصادر الموحدhttp://hdl.handle.net/10576/4025
الملخصOptimization of a fermentation process for bioinsecticides production by Photorhabdus temperata strain K122 was investigated into fully controlled 3-L fermenter using an optimized medium (OM). Development of large-scale inocula showed that the composition of the growth medium greatly influenced the physiological state of P. temperata cells. The effect of pH, agitation and dissolved oxygen concentration (DO) on the growth, culturability and oral toxicity of P. temperata cells were also investigated. Indeed, maintaining the pH at 7 and controlling DO concentration at 50 % saturation throughout the fermentation process, improved biomass production, CFU counts and oral toxicity by 41.1, 35 and 32.1 %, respectively, as compared to cultures carried out in 500 mL shake flasks. At such conditions, 8 g/L glucose fed-batch fermentation, enhanced cell lysis and variants small colony (Vsm) polymorphism appearance. To overcome such limitations, glucose concentration should be maintained at 4 g/L. In this case, P. temperata cells were produced at high cell density and culturability reaching 4.5 and 1.2 × 10(9) cells/mL, respectively. In addition, the stability of the primary form was maintained for a long period in the stationary growth phase and Vsm polymorphism was completely avoided that can be crucial for scale-up the bioprocess of P. temperata bioinsecticide.
راعي المشروعTunisian Ministere de l’Enseignement Superieur, de la Recherche Scientifique (MESRS)
اللغةen
الناشرSpringer-Verlag
الموضوعPhotorhabdus temperata K122
Bioinsecticide
Dissolved oxygen concentration
Formulation
Fed-batch fermentation
Toxicity
Photorhabdus/growth & development
Pest Control, Biological/methods
Insecticides
Bioreactors
العنوانImprovement of Photorhabdus temperata strain K122 bioinsecticide production by batch and fed-batch fermentations optimization
النوعArticle
الصفحات1505-1513
رقم العدد9
رقم المجلد35


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