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AuthorOliva-Olivera, Wilfredo
AuthorCoin-Araguez, Leticia
AuthorSalas, Julian
AuthorLhamyani, Said
AuthorGentile, Adriana-Mariel
AuthorGarcia, Esteban Sarria
AuthorHmadcha, Abdelkrim
AuthorZayed, Hatem
AuthorVega-Rioja, Antonio
AuthorTinahones, Francisco J.
AuthorEl Bekay, Rajaa
Available date2016-03-31T14:00:57Z
Publication Date2015-12
Publication NamePLoS ONE
ResourceScopus
CitationOliva-Olivera W, Coin-Araguez L, Salas J, Lhamyani S, Gentile A-M, Sarria Garcia E, et al. (2015) Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PLoS ONE 10(12): e0144401.
ISSN1932-6203
URIhttp://dx.doi.org/10.1371/journal.pone.0144401
URIhttp://hdl.handle.net/10576/4293
AbstractObjective Adipose Tissue Stromal Cells (ASCs) have important clinical applications in the regenerative medicine, cell replacement and gene therapies. Subcutaneous Adipose Tissue (SAT) is the most common source of these cells. The adult human thymus degenerates into adipose tissue (TAT). However, it has never been studied before as a source of stem cells. Material and Methods We performed a comparative characterization of TAT-ASCs and SAT-ASCs from myocardial ischemic subjects (n = 32) according to the age of the subjects. Results TAT-ASCs and SAT-ASCs showed similar features regarding their adherence, morphology and in their capacity to form CFU-F. Moreover, they have the capacity to differentiate into osteocyte and adipocyte lineages; and they present a surface marker profile corresponding with stem cells derived from AT; CD73+CD90+CD105+CD14-CD19-CD45-HLA-DR. Interestingly, and in opposition to SAT-ASCs, TAT-ASCs have CD14+CD34+CD133+CD45- cells. Moreover, TAT-ASCs from elderly subjects showed higher adipogenic and osteogenic capacities compared to middle aged subjects, indicating that, rather than impairing; aging seems to increase adipogenic and osteogenic capacities of TAT-ASCs. Conclusions This study describes the human TAT as a source of mesenchymal stem cells, which may have an enormous potential for regenerative medicine.
SponsorInstituto de Salud Carlos III/FEDER, EU (PI10/01947, PI13/02628), CTS-7895 from the Consejer?a de Econom?a e Innovaci?n, Ciencia y Empleo, Junta de Andaluc?a/FEDER, EU. R. El Bekay is supported by fellowships from the ISCIII/FEDER, EU "Miguel Servet II" (CPII13/00041). AV-R is under a contract Proyectos de I+D+i para j?venes investigadores from the Ministerio de Econom?a y Competitividad (SAF2014-60649-JIN) and co-funded by Fondo Europeo de Desarrollo Regional-FEDER.
Languageen
PublisherPublic Library of Science
Subject5' nucleotidase
CD133 antigen
CD14 antigen
CD19 antigen
CD34 antigen
CD45 antigen
endoglin
HLA DR antigen
Thy 1 antigen
adipocyte
adipogenesis
adipose tissue
adipose tissue cell
adipose tissue stromal cell
bone development
cell adhesion
cell differentiation
cell structure
colony forming unit F
heart muscle ischemia
osteocyte
stroma cell
subcutaneous fat
thymus
thymus adipose tissue
TitleMyocardial ischemic subject's thymus fat: A novel source of multipotent stromal cells
TypeArticle
Issue Number12
Volume Number10


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