Ultrasensitive Ti3C2TX MXene/chitosan nanocomposite-based amperometric biosensor for detection of potential prostate cancer marker in urine samples
| Author | Hroncekova, Stefania |
| Author | Bertok, Tomas |
| Author | Hires, Michal |
| Author | Jane, Eduard |
| Author | Lorencova, Lenka |
| Author | Vikartovska, Alica |
| Author | Tanvir, Aisha |
| Author | Kasak, Peter |
| Author | Tkac, Jan |
| Available date | 2023-09-18T11:20:59Z |
| Publication Date | 2020-05-01 |
| Publication Name | Processes |
| Identifier | http://dx.doi.org/10.3390/PR8050580 |
| Citation | Hroncekova, S., Bertok, T., Hires, M., Jane, E., Lorencova, L., Vikartovska, A., ... & Tkac, J. (2020). Ultrasensitive Ti3C2TX MXene/chitosan nanocomposite-based amperometric biosensor for detection of potential prostate cancer marker in urine samples. Processes, 8(5), 580. |
| Abstract | Two-dimensional layered nanomaterial Ti3C2TX (a member of the MXene family) was used to immobilise enzyme sarcosine oxidase to fabricate a nanostructured biosensor. The device was applied for detection of sarcosine, a potential prostate cancer biomarker, in urine for the first time. The morphology and structures of MXene have been characterised by atomic force microscopy (AFM) and scanning electron microscopy (SEM). Electrochemical measurements, SEM and AFM analysis revealed that MXene interfaced with chitosan is an excellent support for enzyme immobilisation to fabricate a sensitive biosensor exhibiting a low detection limit of 18 nM and a linear range up to 7.8 μM. The proposed biosensing method also provides a short response time of 2 s and high recovery index of 102.6% for detection of sarcosine spiked into urine sample in a clinically relevant range. |
| Sponsor | The financial support received from, from the Slovak Research and Development Agency APVV 17-0300 is acknowledged. We would like to acknowledge the support from the ERC Proof of Concept grant (No. 825586). This work was made possible by NPRP grant # 9-219-2-105 from the Qatar National Research Fund (A Member of The Qatar Foundation). The findings achieved herein are solely the responsibility of the authors. We would like to acknowledge the support received from the Ministry of Health of the Slovak Republic under the project registration number 2019/68-CHÚSAV-1. |
| Language | en |
| Publisher | MDPI AG |
| Subject | Biosensor MXene Nanocomposite Prostate cancer Sarcosine Sarcosine oxidase |
| Type | Article |
| Issue Number | 5 |
| Volume Number | 8 |
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