• English
    • العربية
  • العربية 
  • Login
  • QU
  • QU Library
  •  Home
  • Communities & Collections
  • Help
    • Item Submission
    • Publisher policies
    • User guides
    • FAQs
  • About QSpace
    • Vision & Mission
    • QSpace policies
Advanced Search
Advanced Search
View Item 
  •   Qatar University QSpace
  • Academic
  • Faculty Contributions
  • College of Health Sciences
  • Biomedical Sciences
  • View Item
  • Qatar University QSpace
  • Academic
  • Faculty Contributions
  • College of Health Sciences
  • Biomedical Sciences
  • View Item
  •      
  •  
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    miR-20b, miR-296, and Let-7f Expression in Human Adipose Tissue is Related to Obesity and Type 2 Diabetes.

    Thumbnail
    Date
    2019-02-01
    Author
    Gentile, Adriana-Mariel
    Lhamyani, Said
    Coín-Aragüez, Leticia
    Clemente-Postigo, Mercedes
    Oliva Olivera, Wilfredo
    Romero-Zerbo, Silvana-Yanina
    García-Serrano, Sara
    García-Escobar, Eva
    Zayed, Hatem
    Doblado, Esther
    Bermúdez-Silva, Francisco-Javier
    Murri, Mora
    Tinahones, Francisco J
    El Bekay, Rajaa
    ...show more authors ...show less authors
    Metadata
    Show full item record
    Abstract
    This study aimed to analyze the potential association of different microRNA (miRNA) molecules with both type 2 diabetes (T2D) and obesity and determine their target genes. Quantitative PCR was used to analyze the miR-20b, miR-296, and Let-7f levels in human visceral and subcutaneous adipose tissues (ATs) in relation to obesity and T2D, miRTarBase 4.0 was used for validation of target genes, and the Protein Analysis Through Evolutionary Relationships (PANTHER) Classification System and the Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to annotate the biological processes of the predicted targets. In AT, miR-20b, miR-296, and Let-7f levels were significantly different between normoglycemic subjects and those with T2D. In visceral adipose tissue, miRNA levels were higher in normoglycemic/obesity samples than in T2D/obesity samples. miR-20b-miR-296 and Let-7f target genes that showed significant differences in both ATs in relation to obesity and T2D were CDKN1A, CX3CL1, HIF1A, PPP2R1B, STAT3, and VEGFA. These genes are known to be principally involved in the vascular endothelial growth factor (VEGF) and WNT pathways. This study provides experimental evidence of the possible correlation between AT miR-20b-miR-296-Let-7f with obesity and T2D, which might involve vascular endothelial growth factor and WNT-dependent pathways that are regulated by six different genes, suggesting a novel signaling pathway that could be important for understanding the mechanisms underlying the AT dysfunction associated with obesity and T2D.
    DOI/handle
    http://dx.doi.org/10.1002/oby.22363
    http://hdl.handle.net/10576/11287
    Collections
    • Biomedical Sciences [‎268 ‎ items ]

    entitlement


    QSpace is a digital collection operated and maintained by the Qatar University Library and supported by the ITS department

    Contact Us | Send Feedback
    Contact Us | Send Feedback | QU

     

     

    Home

    Submit your QU affiliated work

    Browse

    All of QSpace
      Communities & Collections Publication Date Author Title Subject Type Language
    This Collection
      Publication Date Author Title Subject Type Language

    My Account

    Login

    Statistics

    View Usage Statistics

    About QSpace

    Vision & Mission QSpace policies

    Help

    Item Submission Publisher policiesUser guides FAQs

    QSpace is a digital collection operated and maintained by the Qatar University Library and supported by the ITS department

    Contact Us | Send Feedback
    Contact Us | Send Feedback | QU

     

     

    Video