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AuthorNasrallah, Gheyath
AuthorSalem, Rola
AuthorDa'as, Sahar
AuthorAl-Jamal, Ola Loay Ahmad
AuthorScott, Mark
AuthorMustafa, Ibrahim
Available date2019-02-06T07:48:25Z
Publication Date2019-01-01
Publication NameNeurotoxicology and Teratologyen_US
Identifierhttp://dx.doi.org/10.1016/j.ntt.2019.01.004
CitationGheyath Nasrallah, Rola Salem, Sahar Da'as, Ola Loay Ahmad Al-Jamal, Mark Scott, Ibrahim Mustafa, Biocompatibility and toxicity of novel iron chelator Starch-Deferoxamine (S-DFO) compared to zinc oxide nanoparticles to zebrafish embryo: An oxidative stress based apoptosis, physicochemical and neurological study profile, Neurotoxicology and Teratology, 2019, ISSN 0892-0362, https://doi.org/10.1016/j.ntt.2019.01.004.
ISSN0892-0362
URIhttp://hdl.handle.net/10576/11291
AbstractClinically approved iron chelators are effective in decreasing significant transfusional iron accumulation. Starch-Deferoxamine (S-DFO), a novel high molecular weight iron chelator, was produced to increase binding capacity to iron and reduce toxicity. Although its efficacy was established in one small cohort clinical trial, its potential adverse effect was not adequately addressed. We utilized zebrafish model to assess S-DFO toxicity using following assays: mortality, teratogenicity, hatching rate, tail flicking, Acridine Orange staining for apoptosis detection, o-dianisidine staining for hemoglobin synthesis, and the level of Hsp70 as a general stress indicator. Embryos were exposed to different concentrations of S-DFO, Zinc Oxide nanoparticle (ZnO) (positive control), along with untreated control (UC). S-DFO showed no significant mortality nor deformities at all tested concentrations (0.0-1000 μM). Thus, the LC50 is expected to >1000 μM. 100 μM S-DFO treatment did not affect embryo development (as judged by hatching rate); neuromuscular activity (as judged by tail flicking); and hemoglobin synthesis. Neither apoptosis, nor increase in Hsp70 level was noticed upon S-DFO treatment. Our assays demonstrate that S-DFO does not induce cellular or biochemical stress and has no adverse effect on organ development of zebrafish embryos, suggesting its safe use as an iron chelator.
SponsorFunding information: Qatar University, Grant/Award Number: University Internal Grant/QUUG-CAS-DHS-15\16-21.
Languageen
PublisherElsevier
SubjectS-DFO
SubjectIron chelation
SubjectZebrafish
SubjectToxicity
SubjectZnO
TitleBiocompatibility and toxicity of novel iron chelator Starch-Deferoxamine (S-DFO) compared to zinc oxide nanoparticles to zebrafish embryo: An oxidative stress based apoptosis, physicochemical and neurological study profile.
TypeArticle
ESSN1872-9738


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