Show simple item record

AuthorAzzouz, Hichem
AuthorKebaili-Ghribi, Jihene
Authorben Farhat-Touzri, Dalel
AuthorDaoud, Fatma
AuthorFakhfakh, Ines
AuthorTounsi, Slim
AuthorJaoua, Samir
Available date2015-11-05T10:16:13Z
Publication Date2014-08
Publication NamePest Management Science
CitationAzzouz, H., Kebaili-Ghribi, J., ben Farhat-Touzri, D., Daoud, F., Fakhfakh, I., Tounsi, S. and Jaoua, S. (2014), Selection and characterisation of an HD1-like Bacillus thuringiensis isolate with a high insecticidal activity against Spodoptera littoralis (Lepidoptera: Noctuidae). Pest. Manag. Sci., 70:8 1192–1201.
ISSN1526-4998
URIhttp://dx.doi.org/10.1002/ps.3661
URIhttp://hdl.handle.net/10576/3701
AbstractSpodoptera littoralis (Boisduval) larvae are known by their susceptibility to Bacillus thuringiensis subsp. aizawai strains. In order to prevent the appearance of B. thuringiensis (Bt) resistance and to develop economical Bt-based biopesticides, the selection and the characterisation of a B. thuringiensis isolate toxic against S. littoralis larvae and overproducing δ-endotoxins were investigated. Among 124 Tunisian B. thuringiensis isolates assessed against S. littoralis larvae, four isolates showed toxicity similar to and higher than the toxicity of the aizawai strain HD133 and the kurstaki strain HD1 respectively. The plasmid pattern of the selected isolates was similar to that of HD1. Polymerase chain reaction (PCR) analysis using specific primers revealed that these isolates present different gene contents. The only detected gene encoding Spodoptera-specific toxin was cry9. The selected isolates were found to produce bipyramidal and cubic crystals. The assessment of δ-endotoxin production by these isolates showed that BUPM28 produced 43.71 and 80.81% more δ-endotoxin than HD1 and HD133 respectively. The application of osmotic or heat shock stress on the BUPM28 isolate made it possible to enhance δ-endotoxin production by 22 and 23% respectively. On the basis of its potent insecticidal activity and its high level of δ-endotoxin production, the BUPM28 isolate can be considered to be an effective alternative for the control of S. littoralis.
Languageen
PublisherJohn Wiley & Sons, Ltd
SubjectBacillus thuringiensis
SubjectSpodoptera littoralis
Subjecttoxicity
Subjectcharacterisation
Subjectcry genes
Subjectbioinsecticide production
TitleSelection and characterisation of an HD1-like Bacillus thuringiensis isolate with a high insecticidal activity against Spodoptera littoralis (Lepidoptera: Noctuidae)
TypeArticle
Issue Number8
Volume Number70


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record